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Fig. 7. Analysis of basement membrane components in 18.5 dpc tissues. Double-label immunofluorescence analysis (A-D) of nidogen 1 (A,B) and nidogen 2 (C,D) in kidney sections demonstrates strongly reduced levels of nidogen 1 immunoreactivity in the mutants (B), whereas nidogen 2 (D) is normally present. (E,F) Localization of the laminin {gamma}1 chain to basement membrane structures is unaffected through the mutation (F) compared with controls (E). (G) Immunoblot analysis of extracts prepared from lung tissues shows comparable amounts of intact nidogen 1 in mutant lysates. LN, purified laminin-nidogen complex as control. Scale bar: 50 µm in A-F.