Fig. 4. Effects of suppression of Cs-ZicL function on differentiation of (A) endodermal cells, (B) epidermal cells, and (C,D) mesenchyme cells. (A,A') Histochemical detection of endoderm-specific alkaline phosphatase (AP) activity, and in situ hybridization with probe for (B,B’) epidermis-specific gene Cs-Epi1 and (C,C',D,D') mesenchyme-specific gene Cs-mech1. (A-C) Control embryos and (A'-C') embryos developed from eggs injected with Cs-ZicL morpholino. (D,D') Expression of Cs-mech1 in (D) control and (D') experimental embryos developed from eggs injected with Cs-ZicL morpholino, arrested at the 110-cell stage with cytochalasin B. Scale bar (in A) represents 100 µm for all panels.