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Fig. 4. X-gal staining of mouse-chick chimeras sacrificed at E19. Mouse nuclei that have activated the MLC1/3F-nlacZ reporter gene nuclei (blue in A) are detected inside myosin-positive muscle fibers (A,B). Higher magnification in the inset in A shows lacZ-positive nuclei inside fibers. Donor lacZ-positive cells are also detected in myosin-positive cardiocytes (C,D) of mouse-chick chimeras sacrificed at E19. Mouse donor cells that were sorted for PECAM and labeled with DiI were similarly detected in the host chick heart (E), blood vessels (F) and cartilage (G): E, merged DiI (red) and myosin heavy chain (green) fluorescence images; F, merged DiI (red) and desmin (green) fluorescence images; and G, merged DiI (red) and DAPI (blue) fluorescence images.