Fig. 10. Vagal-to-sacral heterotopic graft, E16 gut wholemount. (A) The nerve of Remak (arrowheads) extended from the cloaca, along the gut wall to the duodeno-jejunal junction, where the bile and pancreatic ducts (d) entered the gut. (B) Adjacent to the colorectum, the nerve was large in diameter and distinct ganglia were evident (B, double arrowheads). (A') After vagal-to-sacral grafting, the nerve of Remak extended from the cloaca to the duodeno-jejunal junction, as in controls. However, the nerve was much finer in diameter. (B') Adjacent to the colorectum, although extremely fine in diameter, the nerve of Remak contained small ganglia (double arrowheads). Scale bars: 10 mm in A; 2 mm in B.