Fig. 3. Vagal-to-sacral heterotopic graft, E4. (A) Many QCPN+ cells entered the dorsoventral pathway. This extended from the dorsal neural tube (nt), lateral to the notochord and dorsal aorta (da), and into the dorsal aspect of the gut (g) (arrows, A and B). Occasional immunopositive cells (arrowheads) were present within the mesonephros (m). (B) At the level of the cecal gut, QCPN+ cells encircled the wall of the intestine, and were present within the outer mesenchymal layers of the cecal buds (c). (C) In the hindgut, transplanted vagal NCC accumulated to form the nerve of Remak (R), adjacent to the gut wall, and were present within the undifferentiated layers of the gut mesenchyme (arrowheads). (D) In control embryos, where isotopic and isochronic sacral NC grafts were performed, although occasional cells accumulated to form the rudiment of the nerve of Remak (arrowheads), no QCPN+ cells were observed within the gut wall. Scale bars: 200 µm in A,B,D; 100 µm in C.