Fig. 8. Ret antibody labelling, chick hindgut, E16. (A) Widespread Ret staining (blue) was present within the myenteric plexus (mp), submucosal plexus (smp) and interconnecting nerve fibres spanning the circular muscular layer (arrowheads). (B,B') Following orthotopic grafts of the sacral NC, double stained Ret (blue) and QCPN (brown) cells were present within the nerve of Remak (B), myenteric ganglia (B') and submucosal ganglia (not shown). Double stained cells contained a brown nucleus and blue cytoplasm (arrowheads). Cells immunopositive for Ret and negative for QCPN were also observed (double arrowhead). (C,C') Vagal-to-sacral heterotopic graft. Ret/QCPN double stained cells were present within the nerve of Remak (C), and within submucosal (arrowheads) and myenteric (not shown) ganglia. Scale bars: 50 µm in A; 25 µm in B,C; 10 µm in B',C'.