Fig. 5. (A-F) Schematic (A) and microphotographic examples (B-F) of whole-mount DAB or AP labeled graft derivatives obtained 24 hours post-operatively, illustrating different positions mapped along the anteroposterior dimension of the neural tube (insets in B-F show the positions of the five grafts at 0 hours). The relative position of the labeled cells at stage 10 is shown in A. Note that the circular grafts at stage 4 adopt an elongated configuration because of cell rearrangement along the neural axis (red areas in A) (Schoenwolf and Alvarez, 1989). Representative sections of these whole-mounts are presented in the right-hand column. (G,H) Optic vesicle (from B); (I,J) caudal forebrain and alar mesencephalon (from C). (K,L) rostral midbrain (from D); (M,N) caudal mesencephalon and rhombencephalon (from E); (O,P) spinal cord (from F). Scale bar: 100 µm.