Fig. 4. Expression of cyclic genes in response to reduction of function of her7. The expression of cyclic genes in the presomitic mesoderm of zebrafish at 14 hpf (10 somites) is shown in dorsal view after flat mounting (A-D,G-J,M-P). Expression of dlc (A-D), her1 (G-J) and her7 (M-P). (A,G,M) Two uninjected control embryos with different stage cyclic gene expression; (B-D,H-J,N-P) the classes of increasing severity of cyclic expression domain disruption after injection of 5 ng/µl her7m1. The effect of increasing her7m2 dose on cyclic domain organization is shown in histograms E,K,Q, assayed at 14 hpf (10 somites) for dlc, her1 and her7, respectively. The developmental progression of the disrupted cyclic expression domain phenotype after injection of 7 ng/µl her7m2 is shown in histograms F,L,R for dlc, her1 and her7, respectively.