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Fig. 6. Comm protein is observed in commissural but not ipsilateral cell bodies. (A,B) Comm protein (red), visualised with anti-Comm, co-localises with the cell bodies of the Eagle-positive neurones visualised using eg-GAL4 driving UAS-{tau}GFP and stained with anti-GFP (green). Longitudinal view of a stage 16, anterior towards the left, dorsal upwards. (C,D) Comm protein (green), visualised with anti-Comm, colocalises with the cell bodies of the Sema2b neurones (red), visualised with anti-Myc at stage 16. Anterior is upwards. (E,F) Comm protein (red) does not colocalise with the cell bodies of the ipsilateral vMP2 and dMP2 neurones (green) identified by the 15J2 GAL4 line at stage 16. Arrows indicate Comm colocalisation. Anterior is towards the left.