Fig. 1. Expression of pipe mRNA and a pipe reporter construct in wild-type ovaries. All egg chambers are oriented with the anterior towards the left and dorsal towards the top. (A-E) pipe mRNA distribution. (F-K) pipe-lacZ expression visualized using anti-ß-galactosidase antibodies. (A,F) During early stage 9, pipe is expressed in two domains, a weak anterior domain and a strong posterior-ventral domain. (B,G,C,H) The two domains of expression approach each other during stage 9 and (D,I) fuse, forming a solid ventral stripe at early stage 10. (E) pipe mRNA disappears during stage 10 starting from the posterior pole. (J) Stage 12. (K) Stage 14.