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Fig. 2. Raf loss-of-function and {lambda}top flip-out clones affect pipe expression in a cell-autonomous manner. (A-I) Stage 10 egg chambers (anterior towards the left and dorsal towards the top). Anterior (A) and posterior (D) clones of mutant cells lacking Raf are marked by the absence of GFP (green) expression. (B,E) pipe expression is visualized using a pipe-lacZ construct (red). (C,F) The merged pictures show that pipe is de-repressed in Raf mutant clones (lack of green) located at the dorsal side. De-repression occurs in a cell-autonomous manner and it is not influenced by the position of the clone along the AP axis. (G-I) {lambda}top flip-out clones. (G) {lambda}top-expressing cells are marked by GFP expression (green). (H) pipe expression is visualized using a pipe-lacZ construct (red). (C) The merged pictures show that {lambda}top expression leads to cell-autonomous repression of pipe.