Fig. 5. Expression of MyoD and ALP in MMCs during myogenic and osteogenic differentiation. MMCs were cultured in pmDM for 6 days to induce myogenic differentiation (A) or in the presence of BMP2 for 2 (B) or 4 (C) days to induce osteogenic differentiation. The cells were then subjected to staining for ALP activity (blue) and immunostaining for MyoD (brown). Arrows in B indicate cells expressing both MyoD and ALP. Scale bars: 20 µm.