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Fig. 2. Radiation-induced p21/WAF1 protein expression varies during mammary gland development. Immunohistochemistry using Ab5 anti-p21/WAF1 antisera was performed on BALB/c-Trp53+/+ mammary tissue before (unirradiated, A-D) or 6 hours after (irradiated, E-H) treatment with 5 Gy of whole-body {gamma}-radiation at various stages of post-natal development. Irradiated age-matched, and developmental stage-matched BALB/c-Trp53–/– mice served as controls (I-L). (Magnification 40x).