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Fig. 4. Apoptosis correlates with PCNA expression in irradiated mammary glands. (A) The percentage of cycling cells was determined immunohistochemically by calculating the percentage of PCNA-positive epithelial cells, while the percentage of apoptotic cells was determined by TUNEL assay on virgin (V), early- (P4) and mid-pregnancy (P15) BALB/c-Trp53+/+ mammary tissues before (control) or 6 hours after (irradiated) treatment with 5 Gy of whole-body {gamma}-radiation. Asterisks indicate values statistically different from unirradiated controls (P<0.05). Error bars represent s.e.m. (B) Immunohistochemistry was performed using PC10 anti-PCNA antibody (i-iii) while apoptosis was measured by TUNEL staining (iv-vi) on virgin (V), early- (P4) and mid-pregnancy (P15) BALB/c-Trp53+/+ mammary tissues 6 hours after treatment with 5 Gy of whole-body {gamma}-radiation. (Magnification 40x).