Fig. 2. Representative wholemounts of mammary glands from wild-type and Vdr knockout mice. (A,B) Inguinal mammary glands from 6-week-old virgin female wild-type (A) and Vdr knockout (B) mice maintained on the high Ca²⁺ diet were fixed in Carnoy’s and stained with Carmine Alum to visualize ductal development. Arrows indicate terminal end buds. Note the greater ductal extension and density and more numerous end buds in the gland from the Vdr knockout mouse compared with the wild-type mouse. The darkly stained round object within the ducts is the inguinal lymph node. Scale bar: 2 mm.