Fig. 5. Laminin immunoreactivity in colobomatous eyes. Embryos infected at stage 15-18 (E3) were processed immunohistochemically at stage 29 (E6) with antibodies against laminin. (A,B) Alkaline phosphatase-infected eye. In the area where the choroid fissure is closing, laminin immunoreactivity is present only at the vitreal edge of the retina (top) and at the choroidal surface of the pigmented epithelium (bottom). (C,D) Noggin-infected retinas. The choroid fissure has not closed in an area similar to that depicted in A, B. Laminin immunoreactivity is observed in the region between the two opposing surfaces of the retina and RPE in this area (arrow, C). Scale bars: 50 µm.