Fig. 5. EH cells are not the sites of ectopic nos expression. (A,C) Confocal images of CNS from third instar larvae carrying a UAS-GFP reporter transgene and either the GAL4^109-68 (A) or HD44A-GAL4 (C) driver, immunostained with anti-EH antibody. The merge of anti-GFP immunofluorescence (green) and anti-EH antibody staining (red) is shown. In both cases, cells that lie below the EH cells express GFP (not readily visible in these sections) but the EH cells (arrow) do not. (B,D) Confocal images of CNS from third instar larvae carrying UAS-nos-tub3'UTR transgene and either GAL4^109-68 (B) or HD44A-GAL4 (D) transgenes, immunostained with anti-EH antibody (red). EH cells, their processes and production of EH are not affected when nos is ectopically expressed by these drivers.