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Fig. 5. BrdU incorporation in cultured male germ cells. (A) BrdU-positive cells at day 5 of culture showed large nuclei. (C) At day 8, the positive nuclei of approximately the same size as those of the mature sperm were present (arrowhead). (E) BrdU-positive flagellated sperm were found on day 9. (B,D,F) Control at day 5 (B), at day 8 (D) and normal mature sperm (F) subjected to BrdU detection. Scale bar: 10 µm.