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Fig. 6. Overexpression of unc-53 in muscle cells. In body wall muscle cells, overexpression of unc-53 results in over-extension along the longitudinal axis. (A,C) Wild-type body wall muscle cells visualised with the punc-54::GFP reporter pPD93.48, at the comma (A) and three-fold (C) stages. (B,D) Transgenic embryos carrying the construct punc-54::unc-53 (pTB113) immunostained with the anti-UNC-53 monoclonal antibody MAb 16-48-2 at the comma (B) and three-fold stages (D). Arrows mark cell extremities. (E) Wild-type punc-54::GFP L1 larva and three-fold embryo visualised with DIC optics. (F) Mutant punc-54::unc-53 L1 larva showing severe morphological defects in the posterior body. Scale bars: 10 µm.