Fig. 1. Embryonic germ cell-less (grandchildless) and abdomenless phenotypes of prt^gs mutant embryos. (A) Wild type, early cellular blastoderm embryo with embryonic germ cells at the posterior at 25°C, as shown by staining with anti-Vasa antibody, and (B) embryo without embryonic germ cells from a prt^gs/prt^gs mother. (C,D) Cuticle preparation of wild-type and mutant, prt^gs/prt^gs female originated embryo at 18°C. (C) Wild-type embryo with normal abdominal segmentation, and (D) a mutant embryo from prt^gs/ prt^gs mother with a weak abdomenless phenotype. All embryos oriented anterior leftwards, dorsal upwards.