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Fig. 6. Marker analyses of neuronal differentiation. (A-C) NEX expression at E15.5; (D-F) Lhx2 expression at E15.5; (G-I) RC2 staining at E16.5; (J-L) GFAP staining at E18.5. (A,D,G,J) Wild-type, (B,E,H,K) Emx2–/– and (C,F,I,L) Emx1–/– Emx2–/– cortices. Scale bars, 100 µm.