Fig. 2. Development, proliferation and differentiation in the cerebellum of En2cre; Pten^LoxP/LoxP mice. (A,B) enlarged cerebellar anlage and increased cell size (inset) of neural precursors at embryonic day 15.5 in En2cre; Pten^LoxP/LoxP (B) versus controls (A). (C,D) Immunostaining for BrdU revealed reduced proliferation in mutant cerebella at embryonic day 15.5 (D), compared with littermate controls (C). (E,F) Phosphorylated AKT is highly expressed in the EGL and IGL of the developing wild type cerebellum and in all regions of the mutant cerebellum. (G,I) Widening of the Math1-expressing outer EGL (G, arrowheads) and of the p27-expressing inner EGL (I, arrowheads). Littermate controls are shown in H and J; blue arrowheads in J indicate neurons strongly positive for p27. Scale bar: 400 µm in A,B (60 µm in inset); 50 µm in C,D; 660 µm in E,F; 100 µm in G,H; 200 µm in I,J.