Fig. 6. Cell density, apoptosis and proliferation in the developing cerebellum of En2cre; Pten^LoxP/LoxP mice and controls at E15.5 and postnatally (P1). Although cellular density is not greatly increased at E15.5 (A), the absolute number of cells (C) in the midsagittal cerebellum is almost twice as much in mutant brains, because the total area is increased in mutants (B). Apoptosis is reduced in E15.5 mutant cerebella, compared with controls (D,E), but there is also reduced proliferation in mutant cerebella at days E15.5, P1 and P9 (F) in mutant versus control cerebella.