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Fig. 3. Sperm-specific expression of mouse PLC{zeta}. (A) Northern blot analysis of PLC{zeta} transcript distribution in mouse. Lanes from left to right: RNA standard markers, brain, heart, kidney, liver, lung, skeletal muscle, spleen, testis (2 µg polyA+-RNA/lane). Molecular weight markers in kb are on the left. (B) Immunoblot analysis of PLC{zeta} protein distribution in mouse. Left to right: brain, heart, kidney, liver, lung, skeletal muscle, sperm (50 µg protein/lane). Molecular weight markers in kDa are on the right. (C) Polymerase chain reaction detection of PLC{zeta} in cDNA from mouse spermatid and mouse testis devoid of spermatids. Left to right: DNA markers (2.0, 1.6, 1.0, 0.8, 0.6 and 0.5 kb, top to bottom), spermatid cDNA (10 ng), testis cDNA (10 ng), blank (no DNA) and positive control (1 ng PLC{zeta} plasmid).