Development -- Saunders et al. 129 (15): 3533 Figure IG5

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 5. In vitro fertilisation consistent with PLC ${zeta}$ -induced Ca²⁺ oscillations. Ca²⁺ changes in fura-red loaded mouse eggs that were either (A) in vitro fertilised with mouse sperm, or microinjected with cRNA encoding (B) PLC ${zeta}$ at 0.02 mg/ml; (C) PLC ${delta}$ 1 at 2 mg/ml; (D) ^PHPLC ${delta}$ 1 at 2 mg/ml (PH domain-deleted PLC ${delta}$ 1); (E) ^D210RPLC ${zeta}$ at 2 mg/ml. (A-I,B-I) Expanded traces of the longer-duration, first Ca²⁺ transient taken from A,B, respectively. (F) Autoradiograph following SDS-PAGE of [³⁵S]-labelled protein expressed in vitro from cRNA, lanes from left to right, of ^D210RPLC ${zeta}$ , PLC ${zeta}$ , ^PHPLC ${delta}$ 1 and PLC ${delta}$ 1 corresponding to predicted protein sizes of 74, 74, 70 and 85 kDa, respectively.