Fig. 8. Activation and embryo development to blastocyst in PLC-injected mouse eggs. (A) Mouse eggs were either microinjected with PLC cRNA (0.02 mg/ml), or parthenogenetically activated with strontium (5 mM, 4 hours) or fertilised with sperm in vivo, then placed in a 5% CO₂ incubator at 37°C. Percentage of eggs reaching the two-cell stage after 24 hours, and morula/blastocyst stage after 96 hours, was recorded for each treatment. Number of microinjected eggs is shown above each condition. (B) Micrographs illustrating mouse embryos at the two-cell stage (left) and blastocyst stage (right), at 24 hours and 96 hours, respectively, after egg microinjection with PLC cRNA (0.02 mg/ml). (C) Micrograph illustrating mouse egg 24 hours after microinjection with ^D210RPLC cRNA (0.02 mg/ml).