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Fig. 1. Tracheal phenotype following elB overexpression. (A) Wild-type stage 14 embryo stained with anti-lumen antibody. Note the visceral branch (VB, arrow). (B) In btl-Gal4/EP-elB the VB is not formed in most metameres (arrow). (C) Wild-type stage 14 embryo stained with anti-Trh antibody, marking the tracheal nuclei. Note the presence of five or six nuclei in the dorsal branch formed by tracheal pit 3. (D,D') After tracheal misexpression of ElB, embryos were stained with anti-ElB antibody. Note the nuclear staining and the presence of seven nuclei in the dorsal branch (DB) formed by tracheal pit 3 (arrow). The stalled visceral branch cells remain in the transverse connective (arrowhead). (E) The number of nuclei in the dorsal branch was quantitated in wild type (red bars, n=30), ElB overexpression (green, n=52) and elB mutant embryos (blue, n=54). (F,G) btl-Gal4/UAS-elB embryos were stained with anti-ElB antibodies. Note the reduced visceral branches (arrowhead) and the extended dorsal branches (arrow).