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Fig. 4. Expression of ElB. (A) At stage 11, elB RNA is detected in all tracheal pits. (B) By stage 12, expression is reduced in the central part of the pit, and retained in the dorsal branch and lateral trunks. (C) At stage 13, expression is detected only in the lateral trunk, most prominently in the first metamere. (D) Insertion of a Gal4 element upstream to elB induced (at stage 14) expression of UAS-elB in the lateral branches (arrowhead), but not in the visceral branch or dorsal trunk. Red, anti-ElB; green, 2A12 (which marks the tracheal lumen). (E) At stage 16 the expression of UAS-elB in the spiracular branch, transverse connective, lateral trunk and ganglionic branch was observed. (F) At the same stage, the dorsal branch fusion cells also expressed ElB (arrows).