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Fig. 4. Detection of EphB receptors in explanted trunk neural crest cells. Early outgrowths (A,B) and melanoblasts (C,D) were incubated with ephrin-B1-Fc (A,C) or Fc (B,D) proteins and visualized with anti-human Fc antibody coupled to alkaline phosphatase. Control cultures display no staining (B,D). Ephrin-B1-Fc bound to both early outgrowths (A) and melanoblasts (C), indicating that the receptor remains expressed in vitro.