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Fig. 5. Effect of ephrin-B1-Fc on the migration of neural crest cells into the dorsolateral pathway in whole-trunk explants. Ephrin-B1-Fc (A,C), or Fc alone (B,D) were added to stage-12 (A,B) and stage-18 (C,D) explants and the pattern of neural crest migration was visualized by anti-HNK-1 antibody staining. (A) Addition of soluble ephrin-B1-Fc to stage-12 trunk explants results in the inappropriate migration of neural crest cells (arrows) into the dorsolateral pathway. Control explants (B) show the typical pattern of neural crest cell migration through the ventral pathway (arrow). (C) Addition of soluble ephrin-B1-Fc at the onset of melanoblast migration (stage 18) results in the lack of cells in the dorsolateral pathway. Migration of melanoblasts (arrows) along the dorsolateral pathway is normal in control explants (D).