Fig. 1. Sequence of the cf50 gene. The sequence starts with genomic DNA; the 5' end of known cDNA sequence starts at nt 28 and continues to 83 nt past the stop codon, and is an exact match to the genomic sequence. The first ATG is at nucleotide 25. The N-terminal amino acid sequence obtained from the purified 50 kDa protein is indicated with a heavy underline. Two potential N-linked glycosylation sites (Alexander, 1997) are indicated by shaded boxes. Vertical arrows mark the beginning and end of the sequence expressed in bacteria and then used to immunize rabbits for antibody production. An arrow pointing to the left over nucleotide 260 marks the 3' end of the region that was replaced with a blasticidin resistance cassette to make a gene disruption transformant; the 5' end of the region that was replaced was 681 bp upstream of the first ATG. A double underline indicates a potential AAUAAA poly(A) addition signal. The GenBank accession number is AF405695.