Fig. 8. Distribution of endogenous MSY4 and transgenic MSY4-HA in a sucrose gradient. Testis extract was sedimented on a 15% sucrose (w/w) gradient, the absorbance at 254 nm was recorded and 12 fractions collected. Sample 1 is the top of the gradient. Prominent peaks are labeled on the A₂₅₄ graph. Single ribosome subunits are labeled 40S and 60S, monosomes as 80S/M, disomes as D and trisomes as T. Protein from each fraction was run on a 9% SDS denaturing gel, transferred to nitrocellulose and probed with MSY4 antibody (upper panel) and then HA antibody (lower panel). The location of the 51 kDa standard is indicated on the right.