Fig. 10. Embryos with severe AS-Hro-eve MO phenotype produce kinked germinal bands. Fluorescence images of an embryo in which the left N teloblast had been injected with AS-Hro-eve MO and RDA (red), and the right N teloblast with generic control MO and RDA, at early stage 7. The resultant embryo was fixed and counterstained with Hoechst 33258 (pseudo-colored green in A and D); the animal pole (prospective dorsal) is upwards. (A) A roughly posterior (P) view of the embryo reveals the labeled n bandlets at the leading edges of the left and right germinal bands. While the control germinal band (circle, arrowhead) projects equatorially, the experimental germinal band (square, arrow) makes a marked dorsal deflection. (B) Lateral view of the right germinal band shows that it projects along the equator of the embryo. (C) Lateral view of the left germinal band shows the dorsally directed kink. (D) An obliquely ventral view shows the anterior (A) ends of the labeled bandlets within the partially formed germinal plate. The lineage tracer is brighter within the control bandlet, suggesting that the volume of the control injection was greater than that of the AS-Hro-eve MO injection. Scale bar: 100 µm.