Fig. 3. Semi-quantitative RT-PCR analysis of Hro-eve expression. Ethidium bromide-stained gels (below) show Hro-eve and 18S rRNA bands from various developmental stages (0 denotes oocyte; e, early). The extent of amplification (23 cycles for 18S rRNA and 30 cycles for Hro-eve) was chosen empirically to avoid saturation of the amplified bands. The graph (above) shows the average of the intensity of the Hro-eve bands after normalizing by the intensity of the corresponding 18S rRNA band and plotting relative to stage 10, from five different experiments. Error bars indicate the standard deviation of the mean.