Fig. 6. Dynamic pattern of late Hro-eve expression in ganglionic neurons. Bright-field (Nomarski optics) images of embryos processed by in situ hybridization for Hro-eve. (A) Lateral view of a stage 9 embryo. In anterior, developmentally advanced segments, the spots of Hro-eve expression show segmental periodicity (arrows), while in posterior, less advanced segments, there are two spots per segmental repeat (yoked arrowheads and arrows). (B-D) Ventral views of anterior midbody ganglia (M2-M5) at three different stages; note that these Hro-eve transcripts exhibit cytoplasmic localization; transcripts are excluded from nuclei. (B) At mid stage 9, there are distinct anterior and posterior spots of expression in each hemiganglion (yoked arrowheads and arrows, respectively). Inset: enlarged image of the adjacent cell shows cytoplasmic staining and unstained nucleus. (C) By late stage 9, anterior expression has ceased in anterior segments, whereas the posterior spot is still present in all three segments. (D) By late stage 10, anterior expression is completely gone, but the posterior spots (arrows) remain. Scale bar: 100 µm in A; 50 µm in B-D; 20 µm in inset.