Fig. 9. Embryos with severe AS-Hro-eve MO phenotype produce hairpin loops in germinal plate. (A) Bright-field image showing a lateral view (ventral is downwards, anterior towards the left) of the posterior portion of an embryo in which an N teloblast had been injected with AS-Hro-eve MO and RDA at early stage 7; the embryo was processed for ganglionic Hro-eve expression at stage 10 (inset shows the whole embryo). Note that the pattern of Hro-eve positive spots demonstrates a prominent dorsal excursion (arrow). (B) Fluorescence image of the same specimen shows that the labeled bandlet contains a dorsally directed hairpin loop in this region (arrow indicates same point as in A). Scale bar: 50 µm in A,B; 175 µm in the inset.