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Fig. 7. Differentiation and mineralization of the skull vault. (A,B) Spp1 expression at E16.5 shows delayed formation of the metopic suture and is absent in the nasal bones in the mutant (arrow). (C-F) Alizarin-stained P14 skeletal preparations, showing premature fusion of the medial part of the coronal suture in the Fgfr2IIIc mutant. cs, coronal suture; ls, lambdoid suture; ss, sagittal suture.