Fig. 5. When array disruption occurs, processes that link array cells are still observed and contain microtubules throughout. (A) Double labelling for ChAT (green) and ß-tubulin (red) of a region of the GCL cholinergic array after treatment with MAP2 antisense. (A) Stack of confocal sections. (B-D) Confocal section through A (0.5 µm) , showing the double labelling (B) and ChAT (C) and ß-tubulin (D) immunoreactivity. Although the array cells are irregularly spaced, the ChAT-positive processes linking array cells contain microtubule throughout, as revealed by ß-tubulin immunoreactivity (e.g. the processes linking cells 2-3 or 3-5).