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Fig. 3. FGF3 and FGF8 are required for r5 and r6 development. (A) In situ hybridization of various markers (in blue, named on the left) in wild-type (a,e,i,m,q), fgf3-MO (b,f,j,n,r), fgf8- (c,g,k,o,s) and fgf3-MO; fgf8- (d,h,l,p,t) embryos at 18 somites (a-h) and 20 somites (i-t). Markers in red are pax2a at the MHB (a-t) and krox-20 in r3 and r5 (e-t). In Figs 3, 4 and 8, wild-type and fgf3-MO embryos are siblings of fgf8- and fgf3-MO; fgf8- embryos, respectively. fgf8- and fgf3-MO; fgf8- embryos show loss of pax2a at the MHB. Dorsal views show anterior towards the left. n>=10 for each marker for each ‘genotype’. Scale bar: 50 µm. hb, midbrain-hindbrain boundary; r3, rhombomere 3; r5, rhombomere 5. (B) In situ hybridization of various markers (in blue, named on the left) in wild-type (a,e,i,m,q), fgf3-MO (b,f,j,n,r), fgf8- (c,g,k,o,s) and fgf3-MO; fgf8- (d,h,l,p,t) embryos at bud stage (a-d), five somites (e-l) and three somites (m-t). Markers in red are pax2a at the MHB and krox-20 in r3 and r5. pax2a at the MHB is still expressed, yet weakly and more broadly, in fgf8- and fgf3-MO; fgf8- embryos at these stages. Dorsal views show anterior towards the left. n>=7 for each marker for each ‘genotype’. Scale bars: in a, 50 µm in a-l; in M, 50 µm in m-t. mhb, midbrain-hindbrain boundary; r1-r7, rhombomeres 1-7; fb, forebrain.