Fig. 4. FGF3 and FGF8 are required for neuronal development in r5 and r6. Confocal images of 48 h hindbrains stained with RMO-44, labeling reticulospinal neurons (A-D), anti-Islet, labeling motoneurons (E-H) or zn-8, labeling nVI and nIX motoneurons (I-L). Ventral views show anterior towards the top (A-H) or towards the left (I-L). Arrows in A,D indicate T interneurons. The large Mauthner neurons (M in A) are in r4. Arrows in E-H point to the rostral extent of nX neurons. n
10 for each marker for each ‘genotype’. Scale bar: 50 µm. r2-r7, rhombomeres 2-7; V, nV neurons; VI, nVI neurons; VII, nVII neurons; IX, nIX neurons; X, nX neurons.