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Fig. 4. Ephrin-A5 expression within primary somatosensory and motor cortex at P6 and EphA receptor labeling on E14 thalamic axons in vitro. (A) Serial coronal sections of the right hemisphere (medial is left) stained with Cresyl Violet, ephrin-A5 in situ hybridization and cytochrome oxidase histochemistry. The boundaries between the barrel field of the somatosensory cortex (S) and the motor cortex (M) are indicated by the arrowheads. Bars show the boundaries between cortical layers 4, 5 and 6. (B) Coronal section through the barrel field of S1 stained with Hoechst and EphA3-Fc fusion protein. (C) Phase-contrast and bright-field images of ephrin-A5-AP stained thalamic axons.