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Fig. 8. (A) Outgrowth of thalamic explants confronted with parallel membrane stripes from cortical layer 4 (L4) and cortical layer 5 (L5). (B) Distribution of thalamic axons on membrane stripes from cortical layer 4 (black bars) and cortical layer 5 (white bars) under control conditions and after PI-PLC treatment of one type of membrane. Bars indicate the percentage of axons growing on one type of stripe. Results demonstrate that thalamic axons preferentially extend on their target membrane stripes, and this choice can be blocked by treatment of layer 5 membranes with PI-PLC enzyme. n, number of pairs of stripes analyzed. Scale bar: 100 µm.