Fig. 3. PtdInsP₃ levels control the range of growth and size. (A) Quantification of the eye size of the various mutants. Measurements were made from SEM images by calculating the longest distance of a side view of the eye from dorsal to ventral plus anterior to posterior (n=4-7). The same pattern was also seen when head:thorax ratios were determined from a dorsal view (data not shown). (B) Dorsal view SEM of a y w control fly. Bar above C is 200 µm in all cases. (C) Selective loss of PI 3-kinase p110 function in the head using the eyFlp system results in a strong pinhead phenotype. (D) Loss of PTEN function cannot rescue complete loss of PI 3-kinase p110 function in mosaic clones. The genotype of the fly head shown is Pten^2L117/Pten^2L117; PI 3-kinase^null/PI 3-kinase^null. (E) Selectively loss of PI 3-kinase p60 function in the head using the eyFlp system results in a strong pinhead phenotype. (F) Loss of PTEN function rescues to wild-type size complete loss of Pi3K p60 function in mosaic clones. The genotype of the fly head shown is Pten^2L117/Pten^2L117; PI3K p60^null/Pi3Kp60^null.