Fig. 4. Pten regulates size, metabolism and survival in an opposite manner to positive components of the insulin pathway like Chico. (A) A Pten^2L117/Pten^2L100 mutant fly (right) compared to the y w control (left). (B) Pten^2L117/Pten^2L100 mutant flies weigh more than the y w control (data not shown) or to Pten mutants rescued with the Pten genomic rescue construct (n=10). (C) Pten^2L117/Pten^2L100 mutant flies have decreased glycogen and lipids compared to the y w control (data not shown) or to Pten mutants rescued with the Pten genomic rescue construct (n=10). The glycogen data are transformed by one log₁₀ power. (D) Pten^2L117/Pten^2L100 mutant flies are hypersensitive to water-only starvation conditions compared to a starvation resistant chico¹/chico¹ mutant. All assays were performed at least 2 times independently.