Fig. 3. fu mutations affect stalk morphogenesis. All ovarioles are oriented with the germaria towards the right. (A,B) 93-F/+ (A) and fu^JB3; 93F/+ (B) ovarioles double-stained with anti-Fas III antibodies (A,A',B,B', red) and anti-ß-galactosidase antibodies (A',B', green). FIII denotes Fas III. Arrow in B and insert in B' indicate Fas III^low/93F⁺ peripheral somatic cells. (C',D') Expression of the 93F enhancer-trap line after X-gal detection of ß-galactosidase activity in wild-type (C') and fu^JB3 (D') ovarioles. (C,D) Corresponding DAPI nuclear staining. (E-F') A101/+ (E) and fu^JB3; A101/+ (F) ovarioles triple-stained with DAPI (E,F), anti-Fas III antibodies (E',F', red) and anti-ß-galactosidase antibodies (E'-F', green). Arrows in F,F' indicate interfollicular cells arranged in a double row. (G-J) Wild-type (G,I) and fu^JB3 (H,J) ovarioles stained with DAPI. (G'-J') Magnification of the boxed area of G-J germaria, double-stained with DAPI (red) and anti DE-Cadherin antibodies (green). Ovarioles were dissected either from 7- to 8-day-old females (A-D) or from younger 3-4 day old females (E-J). Scale bars: 10 µm.