Fig. 4. fu is expressed in both the germline and somatic cells of the ovary. All ovarioles are oriented with the germaria towards the top or top right-hand corner. (A) Distribution of fu transcripts in a wild-type germarium and stage 2 and 3 egg chambers. (B,B') Two different confocal sections of a wild-type germarium and stage 2-3 egg chambers stained with anti-Fu antibodies. Confocal section in B corresponds to follicular somatic cells (revealed by their ‘honey comb-like’ staining, arrow) and confocal section in B' to germline cells and anterior-most somatic cells. (C,D) Confocal sections of wild-type (C) and fu¹ (D) stage 4 egg chambers stained with anti-Fu antibodies, showing reduced levels of Fu protein accumulation in fu mutant follicular cells compared with wild type. Identical confocal settings and image processing with Adobe Photoshop were used for C,D.