Fig. 8. Reduction in Hedgehog signaling levels leads to abnormal prefollicular cell behavior. All ovarioles are oriented with the anterior-most region towards the top or top right-hand corner. hh^ts2/hh^ts2 (A,A') and hh^AC/hh^ts2 (B,B') ovarioles dissected from 5-day-old females placed at 29°C immediately after eclosion and double-stained with DAPI (A,B) and anti-Hts antibodies (A',B'). Hts is normally present in spectrosomes and fusomes of germline cells as well as in all somatic cells as of germarial region 2a/2b (Lin et al., 1994). In particular, Hts accumulates at high levels in interfollicular cells. Arrows in A',B' indicate somatic cells aggregated at the periphery of the ovarioles. (C-D') DAPI (C,D) and GFP (C',D', green) staining of hsp-flp/+; Act>CD2>Gal4,UAS-GFP/UAS-cos2 ovarioles. Germarium in D' was additionally stained with anti-Hts antibodies (red). (E-F') DAPI (E,F) and GFP (E',F', green) staining of hsp-flp/+; Act>CD2>Gal4,UAS-GFP/UAS-Ci^cell ovarioles. All prefollicular cells and their descendants are GFP⁺ in C' and E', whereas both GFP⁺ and GFP^– prefollicular and follicular cells are present in D' and F'. (C,E) Arrows indicate apposed egg chambers, arrowheads indicate multicyst egg chambers. Scale bars: 10 µm.