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Fig. 6. Colony formation on MS-5 stroma by 24-week fetal bone marrow hematopoietic and endothelial cells. CD34+ CD45+ (A-C) and CD34+ CD45– (D-F) cells sorted from the fetal bone marrow gave rise to colonies of packed rounded hematopoietic cells on MS-5 cells from day 3 up to day 35 of co-culture. Phase-dark cobblestone-shaped colonies also developed underneath the stroma (arrows) from day 7 to day 35 of co-culture. Scale bars: 20 µm.