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Fig. 3. Association of Ring1B, M33 and Rae28/Mph1 with chromosomal DNA. Protein/DNA complex was concentrated by in vivo formaldehyde crosslinking and subsequent cesium chloride isopyknic centrifugation. (A) Chromosomal DNA in each fraction was visualized by Ethidium Bromide staining. {lambda}DNA digested with StyI was used as molecular weight markers. (B) Protein in each fraction was visualized by Coomassie Brilliant Blue (CBB) staining. Localization by western blot analysis of an ER luminal protein, Bip (C), dimethyl-Histone H3 (D), Ring1B (E), M33 (F) and Rae28/Mph1 (G).