Fig. 2. Endocytosis by Ax2 and ts mutants after a 20 minutes preincubation at 22°C or 28°C. (A,B) Fluid phase uptake measured with FITC-dextran with cells harvested from bacterial plates (A) or held overnight in axenic medium at 5x106 cells/ml (B). (C) Cell surface uptake measured with FM1-43. (D) Phagocytosis of fluorescent beads. Each experiment was carried out at least five times, all with similar results; a representative example of each experiment is shown.